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11.
M. H. F. Klinger 《Annals of hematology》1996,73(3):103-112
When prepared and stored as concentrates, platelets undergo a lot of structural, biochemical and functional alterations that
lead to an impaired function after transfusion. Besides signs of activation like disc-to-sphere transformation, extension
of pseudopodes and loss of storage granules, platelets may display a swollen open canalicular system and changes in the structure
of their α-granules. These partly reversible morphological alterations correspond to a deterioration of basic metabolic parameters
and a decrease in the reactivity of stored platelets to weak agonists. All these changes occur to a very different degree
depending on the methods of preparation and storage. With the introduction of acetate-containing additive solutions, the storage
conditions could be greatly improved, and platelets from pooled buffy coats and stored in an acetate-containing medium with
at least 20% autologous plasma show the best structural integrity over 8 days of storage.
Received: 22 December 1995 / Accepted: 28 May 1996 相似文献
12.
针对医院信息系统现状,提出医疗系统虚拟化拟解决的问题,包括高可用性、管理、故障或容灾以及成本问题。从服务器和存储虚拟化两方面阐述如何采用VMware设计基于大数据的医疗系统虚拟化方案并介绍其虚拟集群应用。 相似文献
13.
目的探讨样本放置时间对血清中二氧化碳检测结果的影响。方法随机收集当天住院患者静脉血样本30例,分别于五个不同时间点(5分钟,45分钟,1小时45分钟,2小时45分钟和3小时45分钟)检测血清样本中二氧化碳含量,评估样本放置时间对二氧化碳检测结果的影响。结果 30例样本5个时间点二氧化碳检测结果有统计学意义(P=0.000);30例样本45分钟时二氧化碳检测结果全部满足质量目标,1小时45分钟时满足质量目标的例数有5例,2小时45分钟和3小时45分钟时满足质量目标的例数有0例。结论样本开盖待测时间对二氧化碳检测结果有显著影响;样本放置45分钟内二氧化碳检测结果的偏差百分率能满足基于生物学变异合适水平的总允许误差质量目标。 相似文献
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目的观察分析凝血检验标本采集与处理过程中的质量控制。方法选择凝血检验标本采集与处理强化质控措施实施后(2018年3月至2019年2月)和实施前(2017年3月至2018年2月)在本院行凝血检验的健康体检者各41例,分别作为观察组和对照组。收集两组待检查者的标本采集、处理过程记录,比较两组标本不合格发生率。结果观察组凝血检验标本不合格发生率(2.44%,1/41)显著低于对照组(19.51%,8/41),差异有统计学意义(P<0.05)。结论强化质控措施可有效提升凝血检验标本采集与处理过程规范性,降低不合格标本风险,有助于保障凝血检验准确性和有效性,临床应用价值较高。 相似文献
17.
ObjectivesThis study aimed to simplify the collection, isolation and cryopreservation procedure of human dental pulp stem cells (DPSCs) to ease the establishment of dental stem cell banking.DesignExtracted third molars were collected and stored either in growth medium or in gentamicin-saline (480 μg/ml) for 6, 9 or 12 h. DPSCs were isolated and subjected to cryopreservation by a controlled-rate or rapid freezing method in 5 or 10% DMSO. Flow cytometry and growth pattern of DPSCs before and after cryopreservation were conducted.ResultsRate of contamination by which the extracted teeth were stored in control and gentamicin-saline were 9.1% (N = 33) and 2.3% (N = 43), respectively. Successful cell isolation rate of teeth preserved in gentamicin-saline at 6 h (92.9%) was comparable to those of growth media group (90.3%). At 9 and 12 h, the rates dropped significantly to 75% and 54%, respectively. Cryopreservation by controlled-rate freezing either in 5 or 10% DMSO resulted in a significantly higher percentage of viable cells than by rapid freezing. Cells conserved by controlled-rate freezing in 5% DMSO showed a pattern of growth similar to control unfrozen cells; 10% DMSO significantly deteriorated the growth pattern of the cells. After thawing, DPSCs conserved by controlled-rate freezing still expressed stemness characteristics, although hematopoietic stem cell markers were slightly increased.ConclusionGentamicin-saline was effective in preserving human teeth for DPSC isolation. Controlled-rate freezing in 5% DMSO gave the highest rate of cell viability. This study simplifies the storage conditions and proposes a simple method for cryopreservation of DPSCs. 相似文献
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19.
Peter A. Smethurst 《Platelets》2016,27(6):526-534
A goal of platelet storage is to maintain the quality of platelets from the point of donation to the point of transfusion – to suspend the aging process. This effort is judged by clinical and laboratory measures with varying degrees of success. Recent work gives encouragement that platelets can be maintained ex vivo beyond the current 5 -7 day shelf life whilst maintaining their quality, as measured by posttransfusion recovery and survival. However, additional measures are needed to validate the development of technologies that may further reduce the aging of stored platelets, or enhance their hemostatic properties. 相似文献
20.
《Vaccine》2018,36(46):6902-6910
Accidental freezing of aluminum-based vaccines occurs during their storage and transportation, in both developed and developing countries. Freezing damages the freeze-sensitive aluminum adjuvanted vaccines, through separation of lattice between aluminum adjuvant and antigen, leading to formation of aluminum aggregates, and loss of potency. In this study, we examined Alhydrogel™ ([AlO(OH)]xnH2O, aluminum hydroxide, hydrated for adsorption) stored under recommended conditions, and exposed to freezing temperature until solid-frozen. The main purpose of our research was to determine the destruction areas of the solid-frozen Alhydrogel™ using selected methods of scanning electron microscopy, energy dispersive X-ray spectroscopy, Raman spectroscopy, Fourier-transform infrared spectroscopy and transmission electron microscopy working in diffraction mode. The Zeta potential evaluation, measurements of albumin adsorption power, thermogravimetric analysis and estimation of the mass loss after drying indicated significant structural (physical) and chemical differences between the freeze-damaged and non-frozen vaccine adjuvant. The presented results are important to better understand the type and nature of damages occurring in freeze-damaged aluminum-based vaccines. These results can be used in future studies to improve the temperature stability of aluminum adjuvanted vaccines. 相似文献